Bibliography.
McFarland, Kenneth [Internet] Botany 111 Fall 2013. [cited ADD DATE]. Available from http://botany1112013.blogspot.com/
In text citation.
(McFarland, 2013)
Wednesday, October 16, 2013
Lab Materials
Information to include
in the Materials and Methods section. Rewrite this information in your
style.
Equipment and Software
MicroAquarium™: is a 75 X 50 X 4mm glass chamber designed for
studying small aquatic organisms with a compound or dissecting
microscope. It is made with two 75 X 50 cm microscope slide glasses
sandwiched together with a 2mm space between. It is sealed on three sides
(two short and one long side) with 100% Silicone Aquarium Sealant from
All-Glass Aquarium Co., Franklin, WI– www.all-glass.com. The MicroAquarium™ is stored vertically (open
end up) in a plastic stand with a clear plastic cap over the opening.
Microscopes: Non –
camera compound microscopes: Observations of MicroAquarium™ made using Micromaster by Fisher
Scientific compound microscope at 40X and 100X objectives.
http://www.olympusamerica.com/
Microscopes:
Compound microscopes
with digital cameras: Olympus CH30 (http://www.olympusamerica.com/) or Leitz Laborlux 11, Leitz Laborlux D or
Leitz Wetzlar (http://www.leica-microsystems.com ) with trinocular heads. Images made at 40X,
100X and 400X (long focus objective).
Dissecting Microscope: Olympus S240 with 0.67- 4.0 zoom objective and
trinocular head (http://www.olympusamerica.com/).
Cameras:
Compound Microscopes: Sony Handycam HDR-HC9, HDR-XR500v (http://www.sony.com/index.php), or Canon EOS T3i cameras for digital imaging
and video recording. Cameras mounted to the trinocular head on the Olympus Ch30
and Leitz compound microscopes with to a Martin Microscope MM99 Adapter S/N:
4310 (http://www.martinmicroscope.com/) adapter.
Dissecting Microscope: Infinity 2 – Lumenera Corp www.Lumenera.com 7 Capella Court.
Ottawa, On K2E 8A7 Canada
Computers: Dell Inc. (http://www.dell.com/) Optiplex 755 or Optiplex 980. Operating system
Microsoft Windows XP.
Software: Images processed with Adobe Photoshop Elements 7.0. Adobe Systems Incorporated, 345 Park Ave, San Jose CA, 95110. Videos processed with Pinnacle Studio 12.1. Pinnacle Systems, Inc, 280 North Bernardo Ave Mountain View, CA 94943.
Software: Images processed with Adobe Photoshop Elements 7.0. Adobe Systems Incorporated, 345 Park Ave, San Jose CA, 95110. Videos processed with Pinnacle Studio 12.1. Pinnacle Systems, Inc, 280 North Bernardo Ave Mountain View, CA 94943.
Lab room 507 Hesler environment from October 22 – November 14, 2013
Temperature: range 15.60 – 17.90 C
Light sources: primary lighting provided from 1 – 15 cm, 30
watt cool light fluorescent lamps suspended 150cm above MicroAquarium™.
Incidental lighting from classroom’s 121 cm long 40 watt cool light fluorescent
ceiling fixtures.
Light energy: 3 - 8 PAR* (light energy range – low
value from suspended light fixture, high values with suspended light fixture
and overhead room ceiling lights)
Light cycle: suspended light fixture illumination 24 hrs/day
and ceiling lights illumination at random time periods between 8:00 AM –
12:00PM during duration of experiment.
* A measure of photosynthetic active radiation, between 400 to 700 nanometers, photosynthetic organisms absorb to drive the light reaction of photosynthesis.
* A measure of photosynthetic active radiation, between 400 to 700 nanometers, photosynthetic organisms absorb to drive the light reaction of photosynthesis.
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